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mnng hos cell line  (ATCC)


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    Structured Review

    ATCC mnng hos cell line
    Mnng Hos Cell Line, supplied by ATCC, used in various techniques. Bioz Stars score: 96/100, based on 1082 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mnng+hos+cells/HOS/pmc12560403-67-0-7
    Average 96 stars, based on 1082 article reviews
    mnng hos cell line - by Bioz Stars, 2026-09
    96/100 stars

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    Related Articles

    Cell Culture:

    Article Title: Redox-active vitamin C suppresses human osteosarcoma growth by triggering intracellular ROS-iron–calcium signaling crosstalk and mitochondrial dysfunction
    Article Snippet: .. U–2OS, 143B, Saos-2, and MNNG/HOS cells were obtained from the American Type Culture Collection (ATCC) and cultured in Dulbecco's Modified Eagle Medium (Gibco, Life Technologies, NY, USA) supplemented with 10 % fetal bovine serum (Sigma-Aldrich, MO, USA) and 1 % penicillin-streptomycin (Gibco, Life Technologies, NY, USA) at 37 °C with 5 % CO2. .. For transfections, siRNAs were introduced using Lipofectamine RNAiMAX (13778075, Thermo Fisher Scientific, Inc, Waltham, MA, USA), and DNA plasmids were transfected using Lipofectamine 3000 (L3000001, Thermo Fisher Scientific).

    Article Title: Long non-coding RNA PVT1 encapsulated in bone marrow mesenchymal stem cell-derived exosomes promotes osteosarcoma growth and metastasis by stabilizing ERG and sponging miR-183-5p.
    Article Snippet: The isolation of exosomes was performed in the 3rd to 6th generation of BMSCs. www.aging-us.com 9590 AGING Cell line and cell culture Human osteosarcoma cell lines (MNNG/HOS, Saos-2, and MG-63) were purchased from American Tissue Culture Collection (ATCC). .. MNNG/HOS cells (CRL1547TM) were cultured in the ATCC-formulated Eagle's Minimum Essential Medium (EMEM, 30-2003TM) supplemented with 1% penicillin-streptomycin and 10% exosome-depleted fetal bovine serum (FBS, ATCC, 30- 2020TM) in which the exosomes were depleted by ultracentrifugation. .. Saos-2 cells (HTB-85TM) were cultured in the ATCC-formulated McCoyʼs 5a Medium Modified (30-2007 TM).

    Article Title: Radiopharmaceutical application of TRK-950, an anti-CAPRIN-1 therapeutic antibody
    Article Snippet: HT-29 cells were obtained from ATCC and cultured in McCoy’s 5A medium (Gibco, 36,600-021) with fetal bovine serum and Pen Step at final concentrations of 10% and 1%. .. MNNG/HOS cells were obtained from ATCC and cultured in DMEM medium (Gibco, 31,966-021) with fetal bovine serum and Pen Step at final concentrations of 10% and 1%. ..

    Article Title: Radiopharmaceutical application of TRK-950, an anti-CAPRIN-1 therapeutic antibody.
    Article Snippet: HT-29 cells were obtained from ATCC and cultured in McCoy’s 5A medium (Gibco, 36,600-021) with fetal bovine serum and Pen Step at final concentrations of 10% and 1%. .. MNNG/HOS cells were obtained from ATCC and cultured in DMEM medium (Gibco, 31,966-021) with fetal bovine serum and Pen Step at final concentrations of 10% and 1%. ..

    Article Title: Redox-active vitamin C suppresses human osteosarcoma growth by triggering intracellular ROS-iron-calcium signaling crosstalk and mitochondrial dysfunction.
    Article Snippet: Pharmacological vitamin C (VC) has gained attention for its pro-oxidant characteristics and selective ability to induce cancer cell death.. However, defining its role in cancer has been challenging due to its complex redox properties.. In this study, using a human osteosarcoma (OS) model, we show that the redox-active property of VC is critical for inducing non-apoptotic cancer cell death via intracellular reactive oxygen species (ROS)-iron-calcium crosstalk and mitochondrial dysfunction.

    Modification:

    Article Title: Redox-active vitamin C suppresses human osteosarcoma growth by triggering intracellular ROS-iron–calcium signaling crosstalk and mitochondrial dysfunction
    Article Snippet: .. U–2OS, 143B, Saos-2, and MNNG/HOS cells were obtained from the American Type Culture Collection (ATCC) and cultured in Dulbecco's Modified Eagle Medium (Gibco, Life Technologies, NY, USA) supplemented with 10 % fetal bovine serum (Sigma-Aldrich, MO, USA) and 1 % penicillin-streptomycin (Gibco, Life Technologies, NY, USA) at 37 °C with 5 % CO2. .. For transfections, siRNAs were introduced using Lipofectamine RNAiMAX (13778075, Thermo Fisher Scientific, Inc, Waltham, MA, USA), and DNA plasmids were transfected using Lipofectamine 3000 (L3000001, Thermo Fisher Scientific).

    Article Title: Redox-active vitamin C suppresses human osteosarcoma growth by triggering intracellular ROS-iron-calcium signaling crosstalk and mitochondrial dysfunction.
    Article Snippet: Pharmacological vitamin C (VC) has gained attention for its pro-oxidant characteristics and selective ability to induce cancer cell death.. However, defining its role in cancer has been challenging due to its complex redox properties.. In this study, using a human osteosarcoma (OS) model, we show that the redox-active property of VC is critical for inducing non-apoptotic cancer cell death via intracellular reactive oxygen species (ROS)-iron-calcium crosstalk and mitochondrial dysfunction.

    other:

    Article Title: CD271 antibody-functionalized HGNs for targeted photothermal therapy of osteosarcoma stem cells.
    Article Snippet: Purpose: Cancer stem cells (CSCs) are considered to maintain the vitality of tumor cell populations through self-renewal and infinite proliferation, but their accessibility still under investigation.. In addition, cancer stem cells are more resistant to chemotherapy and radiotherapy compared with common tumor cells.. This study aimed to develop a kind of novel and feasible nanomaterials for targeted photothermal ablation of osteosarcoma stem cells, which could be a promising anticancer strategy.

    Concentration Assay:

    Article Title: The effect of sodium nitroprusside in scaffolds for synergetic gas‐photothermal therapy of osteosarcoma
    Article Snippet: .. Then, MNNG/HOS cells (purchased from ATCC) were inoculated into 24‐well plates and the DAF‐FM DA probe was diluted to a final concentration of 5 μM, and 200 μL of diluted DAF‐FM DA probe was added and incubated for 20 min. Next, the cells were washed and new DMEM with the PCLA‐ SNP composite scaffold was added. .. Finally, the fluorescence was detected by an inverted fluorescence microscope (Nikon). ow nloaded from https://onlinelibrary.w iley.com /doi/10.1002/bm m 2.70011, W iley O nline L ibrary on [12/08/2025].

    Incubation:

    Article Title: The effect of sodium nitroprusside in scaffolds for synergetic gas‐photothermal therapy of osteosarcoma
    Article Snippet: .. Then, MNNG/HOS cells (purchased from ATCC) were inoculated into 24‐well plates and the DAF‐FM DA probe was diluted to a final concentration of 5 μM, and 200 μL of diluted DAF‐FM DA probe was added and incubated for 20 min. Next, the cells were washed and new DMEM with the PCLA‐ SNP composite scaffold was added. .. Finally, the fluorescence was detected by an inverted fluorescence microscope (Nikon). ow nloaded from https://onlinelibrary.w iley.com /doi/10.1002/bm m 2.70011, W iley O nline L ibrary on [12/08/2025].



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